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Genetic Engineering
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How is insulin made?
Genetically Engineered Bacteria
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What must be present in the bacteria's environment for the T7 promoter to turn on transcription of the GFP?
IPTG
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What decade marks the discovery of insulin and penicillin
1920
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What gene is added to the plasmid during genetic engineering so that only the transformed bacteria will grow in the medium?
An antibiotic resistance gene, like amp
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What bacterial structured is typically altered during the genetic engineering process?
Plasmid
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On which plates would you expect the bacteria to grow in this experimental design?
-DNA/-Amp, +DNA/+Amp, +DNA/+Amp/IPTG
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On the pFluoroGreen plasmid, what gene has the function of turning on transcription for the GFP?
T7 promoter
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