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Genetic Engineering

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    MI unit 4
  •   Study   Slideshow
  • What decade marks the discovery of insulin and penicillin
    1920
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  • How is insulin made?
    Genetically Engineered Bacteria
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  • What bacterial structured is typically altered during the genetic engineering process?
    Plasmid
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  • On the pFluoroGreen plasmid, what gene has the function of turning on transcription for the GFP?
    T7 promoter
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  • What gene is added to the plasmid during genetic engineering so that only the transformed bacteria will grow in the medium?
    An antibiotic resistance gene, like amp
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  • What must be present in the bacteria's environment for the T7 promoter to turn on transcription of the GFP?
    IPTG
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  • On which plates would you expect the bacteria to grow in this experimental design?
    -DNA/-Amp, +DNA/+Amp, +DNA/+Amp/IPTG
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