Study

Blood Bank Chapter 8-10

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  • Where should the tube for blood banking specimens be labeled?
    At patient bedside
  • Why is the agglutination reaction phase important in the interpreting of the antibody screen or antibody identification?
    It can Indicate the class of the antibody
  • If an emergency exists and there is not enough time to perform a crossmatch for red cells, what is type and Rh units should be sent?
    O negative units
  • DAT result is as follows: Polyspecific AHG 1+, Anti IgG neg (CC 3+). The cause is likely:
    Anti C3d
  • A group O patient was Xmatched with group B red blood cells. What phase of the Xmatch will first detect this incompatibility?
    Immediate spin
  • What are the two components of the major crossmatch?
    Donor red cells and recipient serum
  • What incompatibilities are detected in the AHG phase of crossmatch? Include class.
    IgG alloantibodies
  • A smooth layer of cells in the bottom of the well is interpreted as a/an ____________ reaction in solid-phase
    Positive
  • If the recipient was transfused within the last 3 mo, how long from the time of collection can a sample be used for XM?
    3 days
  • What does a negative reaction in the MTS Gel Test look like in the gel card?
    Pellet of cells at the bottom of the column.
  • Why is a recipient’s antibody screening performed as a component of compatibility testing?
    Detects red cell alloantibodies
  • What can a crossmatch not insure?
    That there will not be incompatibility between donor cells and recipient.
  • What is the definition of an antibody demonstrating dosage?
    Homozygous red cells were stronger reactions
  • A donor unit with a positive DAT would cause reactions at what phase of the crossmatch?
  • How long following transfusion must the recipient’s sample be stored?
    7 days
  • A compatible crossmatch is: what reaction at IS, 37, AHG and Check cells
    Neg at IS, Neg at 37, Neg at AHG, Pos at Check cells
  • What is the next step in the investigation of a positive DAT test with polyspecific antihuman globulin reagent?
    Repeat the direct antiglobulin test using monospecific anti-IgG and anti-C3
  • When type O blood is not available for transfusion for a type O recipient, what is the next alternative?
    Nothing
  • How many units of red blood cells are required to raise the hematocrit of a 70-kg nonbleeding adult man from 24% to 30%?
    2 units
  • Why are proteolytic enzymes not used in the routine screening for antibodies?
    Some antigens are destroyed by enzymes, which would cause the antibodies to be undetected
  • If not stored in a monitored refrigerator, how long can RBC units remain outside the transf. service to allow reissue?
    30 minutes
  • A crossmatch demonstrating a 2+ agglutination at AHG is interpreted as compatible. T/F
    False
  • Random access is an important feature in selecting an automated instrument. What is random access?
    The ability to accommodate STAT testing
  • What is the immunoglobulin class of antibodies detected in the immediate-spin crossmatch?
    IgM
  • What is the typical specificity of cold autoantibodies?
    I
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