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A group O patient was Xmatched with group B red blood cells. What phase of the Xmatch will first detect this incompatibility?
Immediate spin
A smooth layer of cells in the bottom of the well is interpreted as a/an ____________ reaction in solid-phase
Positive
A crossmatch demonstrating a 2+ agglutination at AHG is interpreted as compatible. T/F
False
What is the next step in the investigation of a positive DAT test with polyspecific antihuman globulin reagent?
Repeat the direct antiglobulin test using monospecific anti-IgG and anti-C3
Why is the agglutination reaction phase important in the interpreting of the antibody screen or antibody identification?
It can Indicate the class of the antibody
What is the typical specificity of cold autoantibodies?
I
Why are proteolytic enzymes not used in the routine screening for antibodies?
Some antigens are destroyed by enzymes, which would cause the antibodies to be undetected
Why is a recipient’s antibody screening performed as a component of compatibility testing?
Detects red cell alloantibodies
What is the immunoglobulin class of antibodies detected in the immediate-spin crossmatch?
IgM
Where should the tube for blood banking specimens be labeled?
At patient bedside
What are the two components of the major crossmatch?
Donor red cells and recipient serum
What can a crossmatch not insure?
That there will not be incompatibility between donor cells and recipient.
If not stored in a monitored refrigerator, how long can RBC units remain outside the transf. service to allow reissue?
30 minutes
If an emergency exists and there is not enough time to perform a crossmatch for red cells, what is type and Rh units should be sent?
O negative units
How long following transfusion must the recipient’s sample be stored?
7 days
When type O blood is not available for transfusion for a type O recipient, what is the next alternative?
Nothing
