Chromatography

A ______________ where the molecules can move. This is always a liquid or a gas.

The distance each substance moves up the plate depends on its _________in the mobile phase and its retention by or adsorption to the stationary phase__

Which type of the chromatography?

____ _______ help to Identify Components in a Mixture

Explain what is meant by the terms ‘mobile phase’ and ‘stationary phase’.

In _________ _______________ the Mobile Phase is a Gas

Look at this diagram of a chromatogram produced using one-way chromatography on a mixture of substances A and B. Calculate the Rf value of spot A.

Components that are ______ _________ in the mobile phase will travel further up the plate, or faster through the column. It’s these differences in solubility and retention by the stationary phase that separate out the different substances.

In_________________________________ the stationary phase is small particles of a solid packed into a column (or tube). This is often silica bonded to various hydrocarbons

State the formula used to calculate the Rf value of a substance.

Essentially large-scale TLC​ Usually used to separate mixture components for further use rather than simple ID.​ Stationary phase often alumina or silical gel (SiO2xH2O)​. Components separated as they travel at different rates

The mobile phase moves ________ or over the stationary phase.

GC can be used to detect the presence and quantity of alcohol in the blood or urine samples of suspected drink-drivers. What do the letters GC stand for?

HPLC is Done Under _________ _____________

A ___________where the molecules can't move. This must be a solid, or a liquid on a solid support.

Chromatography is used to ______________ stuff in a mixture — once it’s separated out, you can often ___________ the components.

In _________ chromatography a solvent such as ethanol (the mobile phase), moves over a piece of paper (the stationary phase).