Genetic Engineering and EOC review | Baamboozle - Baamboozle

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Bacteria grown in contact with one another can exchange a plasmid through a pilus during

Conjugation

Transformation

Transduction

Confirmation

What decade marks the discovery of insulin and penicillin

1920

Which Patient does not have the disease?

Patient B

Patient A

Paient C

What cell structure is targeted by beta lactams?

Cell Walls

Nucleoid

Ribosome

On the pFluoroGreen plasmid, what gene has the function of turning on transcription for the GFP?

T7 promoter

What bacterial structured is typically altered during the genetic engineering process?

Plasmid

Which of these is a characteristic of Gram negative bacteria?

Endotoxins

Stains purple

Lack of porins in cell wall

Thick cell wall

Sequencing DNA and using a database to identify a pathogen involved in an outbreak uses

Microarray

ELISA

Bioinformatics

Technology Hacks

How is insulin made?

Genetically Engineered Bacteria

What is the correct order for an antigen detection ELISA?

Samples, PrimryAntibody, Enz-linked 2ndAntibody, Substrate

Samples, PrimryAntibody, Substrate, Enz-linked 2ndAntibody

Samples, Enz-linked 2ndAntibody, PrimryAntibody, Substrate

Substrate, Samples, PrimryAntibody, Enz-linked 2ndAntibody

What must be present in the bacteria's environment for the T7 promoter to turn on transcription of the GFP?

IPTG

What antibiotic interferes with cell replication by blocking the enzyme needed for DNA coiling?

Tetracyclines

Fluoroquinolones

Beta Lactams (penicillin)

Sulfanomides

Which patient is likely patient zero of the outbreak?

Patient A

Patient C

Patient B

What gene is added to the plasmid during genetic engineering so that only the transformed bacteria will grow in the medium?

An antibiotic resistance gene, like amp

What cell structure is targeted by tetracycline?

Nucleoid

Ribosomes

Cell Wall

Name a reason for increased antibioic resistant bacteria.

Overuse in farming, Not taking prescriptions as prescribed, Overprescribing

On which plates would you expect the bacteria to grow in this experimental design?

-DNA/-Amp, +DNA/+Amp, +DNA/+Amp/IPTG