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Genetic Engineering and EOC review

  •  English    17     Public
    MI unit 4 Medical Interventions
  •   Study   Slideshow
  • What decade marks the discovery of insulin and penicillin
    1920
  •  15
  • How is insulin made?
    Genetically Engineered Bacteria
  •  10
  • What bacterial structured is typically altered during the genetic engineering process?
    Plasmid
  •  15
  • On the pFluoroGreen plasmid, what gene has the function of turning on transcription for the GFP?
    T7 promoter
  •  15
  • What gene is added to the plasmid during genetic engineering so that only the transformed bacteria will grow in the medium?
    An antibiotic resistance gene, like amp
  •  15
  • What must be present in the bacteria's environment for the T7 promoter to turn on transcription of the GFP?
    IPTG
  •  15
  • On which plates would you expect the bacteria to grow in this experimental design?
    -DNA/-Amp, +DNA/+Amp, +DNA/+Amp/IPTG
  •  15
  • Sequencing DNA and using a database to identify a pathogen involved in an outbreak uses
    Bioinformatics
    ELISA
    Microarray
    Technology Hacks
  •  15
  • What is the correct order for an antigen detection ELISA?
    Substrate, Samples, PrimryAntibody, Enz-linked 2ndAntibody
    Samples, PrimryAntibody, Enz-linked 2ndAntibody, Substrate
    Samples, Enz-linked 2ndAntibody, PrimryAntibody, Substrate
    Samples, PrimryAntibody, Substrate, Enz-linked 2ndAntibody
  •  20
  • Which patient is likely patient zero of the outbreak?
    Patient A
    Patient C
    Patient B
  •  20
  • Which Patient does not have the disease?
    Patient A
    Paient C
    Patient B
  •  20
  • What cell structure is targeted by tetracycline?
    Cell Wall
    Nucleoid
    Ribosomes
  •  20
  • What cell structure is targeted by beta lactams?
    Cell Walls
    Nucleoid
    Ribosome
  •  20
  • What antibiotic interferes with cell replication by blocking the enzyme needed for DNA coiling?
    Fluoroquinolones
    Sulfanomides
    Tetracyclines
    Beta Lactams (penicillin)
  •  20
  • Which of these is a characteristic of Gram negative bacteria?
    Thick cell wall
    Lack of porins in cell wall
    Stains purple
    Endotoxins
  •  25
  • Bacteria grown in contact with one another can exchange a plasmid through a pilus during
    Confirmation
    Transduction
    Conjugation
    Transformation
  •  15