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DNA & Protein Synthesis

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  • RNA polymerase read the DNA strand ___' to ___' because new nucleotides can only be added to the ___' end
    RNA polymerase read the DNA strand 5' to 3' because new nucleotides can only be added to the 3' end
  • A spliceosome is a group of enzymes that work together to cut out ______, the unneeded parts of the mRNA, and glue back together the _______.
    Introns; exons
  • A point mutation where a non-wobble base is swapped but causes no change in the amino acid is...
    Conservative missense mutation
  • tRNA is a type of RNA that has what function?
    transfer RNA (tRNA) has an anti-codon that matches the codon on the mRNA. Each tRNA carries a specific amino acid depending on the anti-codon.
  • Chargaff's Rule says that in DNA, Adenine bonds with ______, so they must be equal
    Thymine
  • A point mutation where a nucleotide is swapped but nothing occurs is called a...
    silent mutation
  • mRNA is a type of RNA that has what function?
    mRNA is a copy of a section of DNA that can be translated into an amino acid sequence.
  • Once out of the nucleus, a ribosome reads the mRNA 3 nucleotides at a time. These are called...
    Codons
  • Translate the given mRNA sequence: 5'-AUGACCGUUAGG-3'
    Met-Thr-Val-Arg
  • In DNA, Adenine binds with _______, but in RNA, Adenine binds with _______
    Thymine; Uracil
  • rRNA is a type of RNA that has what function?
    Along with some other proteins, the ribosomal RNA (rRNA) makes up the ribosome that will translate the mRNA sequence into amino acids.
  • Going from mRNA to amino acids is called..
    Translation
  • A point mutation where a nucleotide is swapped and it causes a stop codon to be in place instead is called...
    Nonsense mutation
  • Breaking ATP into ADP and a phosphate group is an example of
    Catabolism
  • The strand of DNA that mRNA is NOT built onto, but will match, is called the
    Coding strand
  • A _______ mutation is when nucleotides are added to the mRNA strand. This causes a frameshift
    Insertion
  • Here is a coding strand of DNA, transcribe it: 5'-ATGCCTAAG-3'
    Because this is the CODING strand, we know that mRNA is build onto the template strand and essentially matches the coding strand (with the exception of U)
  • Going from DNA to mRNA is called...
    Transcription
  • Watson & Crick were the first to develop and fully fleshed out model of DNA. They said the structure of DNA was what?
    A double helix
  • Anabolism can be defined as...
    The building of molecules-like creating PGAL during photosynthesis
  • RNA polymerase is an enzyme that does what?
    Adds complementary RNA nucleotides (A, U, G, or C) to the template strand of DNA to create a single strand of mRNA
  • During elongation, the incoming tRNA molecules enters the ___ site, moving the previous one to the ___ site, and the one before that to the ___ site.
    A, P, E
  • The sugar in DNA is
    deoxyribose
  • A ______ mutation is when one nucleotide is swapped for another. Sometimes this leads to no problems because of the "wobble base"
    Point mutation
  • What are the four different nitrogenous bases found in DNA?
    Adenine, Thymine, Guanine, Cytosine
  • One key difference between a DNA nucleotide and RNA nucleotide lies within the sugars they are built with. What is this difference?
    Deoxyribose does not have a hydroxyl (OH) group on the 2' carbon whereas the Ribose does.
  • If 30% of a DNA sample was found to be Guanine, what are the percentages of the other bases?
    G-30%, C-30%, A-20%, T-20%
  • A point mutation where a non-wobble base is swapped and the amino acid is changed is a...
    Non-conservative missense mutation