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DNA and DNA replication

  •  English    30     Public
    A quiz for testing the basics of DNA structure, function and the replication process.
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  • List the four essential enzymes for DNA replication:
    DNA polymerase, Helicase, Ligase, Primase.
  •  20
  • Apart from enzymes, list two essential ingrediants for DNA replication:
    Free nucleotides, Template strand.
  •  15
  • DNA repliciation is described as being semi-conservative, explain what this means?
    Each new strand produced contain an original template strand and a newly built strand.
  •  15
  • When does DNA replication occur?
    Before mitosis / Interphase - S-phase
  •  10
  • Why does DNA replication occur?
    A second copy of DNA is required for producing the daughter cell in mitosis.
  •  15
  • All living things contain DNA, true or false?
    True!
  •  10
  • Explain why DNA strands have a 5' end and a 3' end
    Each carbon is numbered on a nucleotide. The last carbon on the strand is5th = 5', 3rd = 3'.
  •  20
  • The DNA backbone has weaker bonds than the middle of the strand?
    False!
  •  10
  • Which direction does DNA polymerase add nucleotides?
    5' to 3' direction.
  •  15
  • What do the single stranded binding proteins (SSBP) do?
    Hold template strands in place, prevents DNA strands fronm joining together.
  •  15
  • Explain the difference between leading and lagging strand?
    Leading strand is made faster (template strand: 3'-5'). Lagging strand is made more slowly and in chunks (template strand: 5'-3').
  •  15
  • What are Okazaki fragments?
    Short DNA chunks of the lagging strand.
  •  10
  • The lagging strand requires multiple primers to build, true or false?
    True!
  •  10
  • Assuming 10% of a DNA sample is G, How much is in total is A and T?
    80% (40%A + 40%T = 80% A&T)
  •  10