Which of the following are required in a polymerase chain reaction (PCR)?
RNA polymerase, ligase and primers
DNA polymerase, template strand and ligase
RNA polymerase, template strand and primers
DNA polymerase, template strand and primers
1000 DNA fragments are present at the start of a 5 min PCR cycle. How many after 20 min?
8000
4000
16000
32000
The genetic material in human mitochondria is arranged as:
Linear chromosomes
Circular chromosomes
Circular plasmids
Linear plasmids
What is the name for the basic units which make up strands of DNA?
Nucleotides
Sugars
Phosphates
Bases
What are the DNA base pairing rules?
Adenine to Guanine, Cytosine to Thymine
Adenine to Thymine, Cytosine to Guanine
Adenine to Uracil, Cytosine to Guanine
Adenine to Cytosine, Thymine to Guanine
The two strands of DNA are held together by which kind of bonds?
Hydrogen
Peptide
Strong chemical
Phosphate
Which of these enzymes is required for DNA replication?
Ligase
Dehydrogenase
Restriction endonuclease
Amylase
Why can both strands of DNA not be replicated continuously?
DNA nucleotides can only be added in a 3’ to 5’ direction
There is not enough space for both strands to be copied
DNA nucleotides can only be added in a 5’ to 3’ direction
The DNA polymerase can only act on one strand at a time
PCR is a technique used for what?
The amplification of DNA in livin la vida loca
The amplification of DNA in vitro
The amplification of DNA in limbo
The amplification of DNA in vivo
Which type of RNA carries a copy of the genetic code from the nucleus to the ribosome?
mRNA
zRNA
tRNA
rRNA
