Medical Interventions Fall Final Review | Baamboozle - Baamboozle

English 51 Public

MI Units 1 and 2

Study Slideshow

If a DNA sample contained just one copy of a gene, how many copies would there be after 4 cycles of PCR?

2X4=8

1X2X4=8

1X4^2 = 16

1 X 2^4 = 16

25

What happens in the annealing phase of PCR?

Double stranded DNA separates

Taq polymerase adds nucleotides to the growing strand

Replication of nucleotide sequence

Primers bind to DNA strand

20

What happens in the denaturation phase of PCR?

Primers bind to DNA strand

Double stranded DNA separates

Taq polymerase adds nucleotides to the growing strand

Replication of nucleotide sequence

20

What happens in the extension phase of PCR?

Double stranded DNA separates

Replication of nucleotides

Primers bind to DNA strand

Taq polymerase adds nucleotides

20

When genetically engineering a vaccine, what does the cutting, like scissors?

DNA Ligase

taq polymerase

Restriction Enzymes - same one for viral DNA and plasmid DNA

20

When genetically engineering a vaccine, what 'tapes' the plasmid and the gene of interest together?

Restriction Enzymes

DNA ligase

Taq Polymerase

20

When genetically engineering a vaccine, how do you ensure that the sticky ends will match up on the plasmid and the viral DNA?

Use TWO re enzymes for plasmid and ONE for viral DNA

Use ONE restriction enzyme for plasmid and TWO for viral DNA

Use SAME restriction enzyme, cut plasmid once and DNA twice

Use SAME restriction enzyme, cut plasmid twice and DNA once

25

How do you get a genetically engineered plasmid into a bacteria, to get it to manufature the protein you need for a vaccine?

TRANSDUCTION- use a virus

CONJUGATION-expose the bacteria to another bacteria

TRANSDUCTION - allow the bacteria to take up plasmid

TRANSFORMATION - allows the bacteria to take up the plasmid

25

Cystic Fibrosis

Is not a genetic disorder

Only requires one parent to pass a mutated copy

Requires that a mutated copy be passed from BOTH parents

Is a chromosomal disorder

20

Which phase of PCR requires a cooler temperature?

Denaturation

Extension

Annealing

15

A study that follows subjects (that have been exposed) and controls (that have not been exposed) over time

Exposed - Not Exposed Study

Double Blind Study

Case Control Study

Cohort study

15

What is the purpose of having a control group in a study?

To gain funding for the study

To ensure that the disease is correlated to the risk factor

In case the exposed or disease subjects die

15

When using electrophoresis for genetic testing, why do you pick a restriction enzyme that only cuts where the mutation occurs?

So that you can distinguish between mutated and nonmutated

So that you can discover where in the gene the mutation is

To locate the nonmutated copies

15

Which can detect a neural tube defect?

Amniocentesis

Ultrasound

CVS

15

What do you need in your tubes to run PCR?

DNA samples, Specific Primer, Nucleotides, Taq polymerase

Primary Antibody, Secondary Antibody, Enzyme and Substrate

Restriction Enzymes, DNA ligase and DNA samples

15

Ossicles

6

1

3

7

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