Medical Interventions Fall Final Review | Baamboozle - Baamboozle

These are the Villans of what Chrismas Classic?

Home Alone

What gave Frosty his magic powers to "come to life one day"?

Hat

Santa

Wendy's of course

Broomstick

This is

Rudolf the Red Nose Reindeer

Who gave you 5 golden rings?

My true love

Frosty

Rudolf

Partridge in a pear tree

What process makes copies of DNA from a specific primer?

PCR

ELISA

Electrophoresis

Restriction Digest

What is the role of T cells in the immune response?

activate B cells

make antibodies

consume pathogen and display antigen

identify pathogen

What is contact tracing an important step in an outbreak investigation?

In order to identify those that are possibly infected to isolate them, identify who is NOT vaccinated in order to stop the spread.

Which is a fast, less expensive and easy way to identify or rule out a pathogen accurately?

ELISA

DNA sequencing and BLAST analysis

Gram staining

What is the most ACCURATE way to identify a pathogen?

DNA sequencing and Bioinformatics (BLAST) Data base analysis

ELISA

Gram Staining

Microscopic Analysis

Which one has an outer layer with endotoxins?

B

A

Which one has a THICK layer of peptidoglycan?

A

B

Which one is gram negative?

B

A

_______ interferes with the supercoiling of DNA during DNA replication.

Fluoroquinolones

Beta Lactams (Penicillin)

Sulfonamides

Tetracyline

_______ interferes with folic acid processing.

Sulfonamides

Beta Lactams (Penicillin)

Fluoroquinolones

Tetracyline

_______ interferes with protein synthesis.

Tetracyline

Beta Lactams (Penicillin)

Fluoroquinolones

Sulfonamides

_______ interferes with cell wall synthesis.

Beta Lactams (Penicillin)

Tetracyline

Fluoroquinolones

Sulfonamides

Which bacterial gene transfer method involves a bacteriophage, or virus, transferring DNA to a bacteria?

Transduction

Transformation

Conjugation

Which method of bacterial gene transfer involves the uptake of a plasmid or environmental DNA into the cell?

TRANSFORMATION

TRANSDUCTION

CONJUGATION

Which method of bacterial gene transfer always involves a plasmid being transferred through a pilus of bacteria in direct contact?

Conjugation

Transformation

Transduction

If the original sample has a concentration of 100, what is the final concentration of tube 2?

11

1.23

33

What is the final dilution of tube 2?

1/9

1/3

1/27

What is the tube dilution of each tube?

1/3

1/2

50/100

What is the correct order for an antigen detection ELISA?

Patient Samples, 1ary antibody, 2ary antibody, substrate

1ary antibody, 2ary antibody, enzyme, substrate

Enzyme, Primary Antibody, Secondary Antibody

Substrate, Samples, Enzymes, Substrate

What is the correct order for an antigen detection ELISA?

Patient Samples, 1ary antibody, 2ary antibody, substrate

1ary antibody, 2ary antibody, enzyme, substrate

Enzyme, Primary Antibody, Secondary Antibody

Substrate, Samples, Enzymes, Substrate

Which antibiotic would you prescribe for a patient infected with this strain of bacteria?

Disc E's antibiotic

Disc C's antibiotic

Disc B's antibiotic

Disc A's antibiotic

Which antibiotic is this bacteria most susceptible to?

Disc E's antibiotic

Disc C's antibiotic

Disc B's antibiotic

Disc A's antibiotic

Which antibiotic is this bacteria likely resistant to?

Disc A's antibiotic

Disc C's antibiotic

Disc B's antibiotic

Disc E's antibiotic

Which disc allows researchers to see what bacteria growing around the disc would look like, and ensures that the disc itself does not inhibit growth?

CONTROL - which is a NEGATIVE control

Disc A

Disc B

Disc E

Which of these can cause a sensorineural hearing loss?

Damage to hair cells in cochlea

Damage to tempanic membrane

Damage to ossicles

Damage from eustacian tube infections

This patient has a SEVERE ____________________hearing loss.

Sensorineural

Conductive

This patient has a SEVERE hearing loss at what frequencies?

High

Midrange

Low

Important for BALANCE

7

6

3

4

TYMPANIC MEMBRANE or eardrum

2

7

3

6

PINNA funnels sound waves to ear

1

7

3

6

Where HAIR CELLS (nerves) are found

7

1

3

6

Ossicles

3

1

7

6

What do you need in your tubes to run PCR?

DNA samples, Specific Primer, Nucleotides, Taq polymerase

Restriction Enzymes, DNA ligase and DNA samples

Primary Antibody, Secondary Antibody, Enzyme and Substrate

Which can detect a neural tube defect?

Amniocentesis

CVS

Ultrasound

When using electrophoresis for genetic testing, why do you pick a restriction enzyme that only cuts where the mutation occurs?

So that you can distinguish between mutated and nonmutated

So that you can discover where in the gene the mutation is

To locate the nonmutated copies

What is the purpose of having a control group in a study?

To ensure that the disease is correlated to the risk factor

To gain funding for the study

In case the exposed or disease subjects die

A study that follows subjects (that have been exposed) and controls (that have not been exposed) over time

Cohort study

Case Control Study

Exposed - Not Exposed Study

Double Blind Study

Which phase of PCR requires a cooler temperature?

Annealing

Denaturation

Extension

Cystic Fibrosis

Requires that a mutated copy be passed from BOTH parents

Is not a genetic disorder

Only requires one parent to pass a mutated copy

Is a chromosomal disorder

How do you get a genetically engineered plasmid into a bacteria, to get it to manufature the protein you need for a vaccine?

TRANSFORMATION - allows the bacteria to take up the plasmid

CONJUGATION-expose the bacteria to another bacteria

TRANSDUCTION- use a virus

TRANSDUCTION - allow the bacteria to take up plasmid

When genetically engineering a vaccine, how do you ensure that the sticky ends will match up on the plasmid and the viral DNA?

Use SAME restriction enzyme, cut plasmid once and DNA twice

Use ONE restriction enzyme for plasmid and TWO for viral DNA

Use TWO re enzymes for plasmid and ONE for viral DNA

Use SAME restriction enzyme, cut plasmid twice and DNA once

When genetically engineering a vaccine, what 'tapes' the plasmid and the gene of interest together?

DNA ligase

Taq Polymerase

Restriction Enzymes

When genetically engineering a vaccine, what does the cutting, like scissors?

Restriction Enzymes - same one for viral DNA and plasmid DNA

DNA Ligase

taq polymerase

What happens in the extension phase of PCR?

Taq polymerase adds nucleotides

Primers bind to DNA strand

Double stranded DNA separates

Replication of nucleotides

What happens in the denaturation phase of PCR?

Double stranded DNA separates

Primers bind to DNA strand

Taq polymerase adds nucleotides to the growing strand

Replication of nucleotide sequence

What happens in the annealing phase of PCR?

Primers bind to DNA strand

Double stranded DNA separates

Taq polymerase adds nucleotides to the growing strand

Replication of nucleotide sequence

If a DNA sample contained just one copy of a gene, how many copies would there be after 4 cycles of PCR?

1 X 2^4 = 16

1X4^2 = 16

2X4=8

1X2X4=8