What functional group is found in DNA, participates in energy metabolism and is a carbon single bonded to 3 hydrogens
Methyl
What happens when acid fast bacteria are stained?
primary stain carbolfuchsin is retained during decolorization step because of cell walls waxy lipids, stays red after counterstain of methylene blue
What dyes are used in an endospore stain? What is the procedure? If endospores are present, how do they appear?
primary stain of malachite green with heat, decolorize with water, counterstain with safranin, endospores will be green in red cell
What type of bond holds A hydrogen atom of one nucleotide to a nitrogen or oxygen atom of another nucleotide in DNA together
Hydrogen bond
What is a basic dye? What is an acidic dye?
Basic- chromophore is a cation, basic dye (+) charged, is attracted to the (-) charged cell wall; acidic dyes are (-), chromophore is an anion, repels (-) cell
What color are Gram(-) cells after adding the decolorizer, alcohol acetone? After the counter stain safranin?
Clear after decolorizer and pink after counterstain
Which can provide more energy for a cell and why: ATP or ADP?
ATP has 3phosphate molecules and more energy than ADP, which only has 2
Identify the building blocks and structure of proteins.
Amino acids which is a carbon atom linked to an amino group, carboxyl group, H+ atom and a variable group
What type of bond holds Li+ and Cl- in LiCl together
ionic
Which microscopes give a 3D image of a specimen?
confocal and electron microscopes
What are characteristics of an organic molecule? Give examples
always contains a carbon! Structurally complex, protiens, nucleic acids, lipids...
What is the difference between an Ionic bond and a covalent bond?
Ionic is losing and gaining electron(s) Covalent is sharing the same electron(s)
What functional group helps build organic acids, proteins and lipids, (COOH)
carboxyl
Which microscope could you use to view internal structures of live specimens?
phase-contrast, two-photon, DIC, fluorescence, and confocal microscopes
What type of bond holds together Carbon and oxygen atoms in methanol
Single covalent
using the whiteboard draw the following functional groups; sulfhydyl, ester, ether, carboxyl
SH, COO C2OH4, COOH
Compare and contrast DNA and RNA differ?
RNA contains the sugar ribose, DNA deoxyribose, DNA double strand, RNA single, RNA uses uracil, DNA thyamine; they both use Adenine, cytosil and guanine
Give examples of a polysaccharide
starch cellulose, glycogen
What is a monomer of sucrose?
glucose, fructose
What are endospores
resting cells, resistant to desiccation, heat, and chemicals.
using the whiteboard, draw the following functional groups methyl, aldehyde, ketone, phophate, amino
CH3, COH, CO, PO4, NH2
Why doesn't a negative stain color a cell?
negative stains are acidic-due to repulsion between the negative charges of the stain and the (-) bacterial surface, the dye will not penetrate the cell.
When preforming a gram stain you forgot the decolorizing step, what color would gram (+) bacteria be? Gram (-)?
They would both be purple
List several properties of water that are important to living systems.
ability to act as a universal solvent, cohesion, adhesion,specific heat capacity help support the life processes and habitats of all organisms.
If a reaction is endergonic and anabolic, what type of reaction could it be?
synthesis reaction
What stain would be used to identify mycobacterium and nocardia?
acid fast
Antacids neutralize acid by the following reaction. Mg(OH)2 + 2HCl ® MgCl2 + H2O Identify the acid, base, and salt.
Mg(OH)=base; HCl=acid; MgCl2=salt
What gives electron microscopy greater resolution?
shorter wavelengths
What happens when non acid fast bacteria are stained using acid fast staining technique?
primary stain makes cells red but the cell walls lack waxy lipid component, is washed away by the decolorization step, then the counterstain makes cells blue
Your experience on this site will be improved by allowing cookies.