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Blood Bank Chapter 2-4
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What does "anti human" mean as it pertains to AHG?
Antibodies in AHG were created by animals "against" human antigen.
Where will I find the limitations of my AHG reagent?
Product insert/ package insert
What temperature is used for incubation in the indirect antihuman globulin test?
37 degrees C
What immunoglobulin class reacts best by antiglobulin testing?
IgG
What is the last step in a negative antibody screen?
Adding and reporting check cells
If ionic environment is reduced...what is increased?
Antibody uptake onto the red cells
Why incubate at 37C?
Temperature acts by increasing the rate of reaction
What is detected by agglutination or hemolysis at the completion of testing?
Antigen-Antibody reactions
Does temperature of reaction affect agglutination? What temp do we find clinically significant reactions?
Yes, clinically significant reactions occur at 37C
What is zeta potential?
Force of repulsion between RBC's
What is the term that describes the expression of most blood group inheritance?
Autsomal co-dominant
What term is used when red cells with the genotype MM react stronger with anti-M, than red cells with genotype MN?
Dosage effect
What information is provided by hemagglutination testing for red cell antigens?
Phenotype
Where is a gene in a 'cis' position located?
On the same chromosome
What source of antibody is selected to determine the specificity of a red cell antigen in a patient sample?
Commercial antisera
What characteristic is associated with polyspecific AHG?
It contains antibody specificity for IgG and C3d
Why are group O red cells used as a source for commercial screening cells?
ABO antibodies do no react with group O
Part of daily QC in blood bank laboratory is the testing of reagent antisera with corresponding antigen positive and antigen negative red cells. What does this procedure ensure?
Antibody specificity and potency
What phase of testing used IgG sensitized RBC's?
Antiglobulin testing
What is the immediate spin phase?
Immediately following centrifuging room temp
What does sensitized mean?
Immunoglobulin or complement attached to cell
What temperature does IgM react best at?
Room Temp 20-22 degrees C or lower
What does the antibody screen test for?
alloantibodies
What stimulates the primary immune response in blood?
Exposure to antigen
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