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Blood Bank Final Exam
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Why is the agglutination reaction phase important in the interpretation of the antibody screen or panel?
It will generally indicate the immunoglobulin class of the antibody
A patients antibody screen is positive, what information will be important for us to know when we begin investigating this result.
Previous history of pregnancy or transfusion
What is the immunoglobulin class of most Rh system antibodies?
IgG
Why is important to determine the presence of the D antigen in women during pregnancy?
D negative moms should be given Rhogam to prevent possible formation of anti D against baby
What percentage of the population is D negative?
Approx 15%
Most naturally occuring ABO system antibodies fall into which immunoglobulin class?
IgM
What is the likely consequence of giving a Group O recipient a unit of Group B PRBC's?
Intravascular hemolytic transfusion reaction
What forward typing antisera is used to confirm group O units before placing them in inventory?
Anti A,B
According to Landsteiner's Law, what ABO antibody will be detected in a group A individuals serum/plasma?
Anti B
What ABO phenotypes will agglutinate in the presence of anti A,B that is produced by group O individuals?
A and B
An O neg mom gives birth to O positive baby. The rosette test is positive. What is the next step?
Kleihauer-Betke test should be performed to quantify the fetal maternal hemorrhage
Name the term used to describe a genetic state in which no detectable trait exists.
Amorph
Why are antibodies to Lub antigen not commonly detected?
Lu b antigen is of high incidence/frequency.
RBC's are phenotyped as Fy(a+b+). Are these cells homozygous or heterozygous?
Heterozygous
Your patient has a positive antibody screen. The antibody identification panel identifies an anti-E. What is the next required step prior to releasing compatible units?
Compatible units must still be phenotyped for E antigen to assure they are negative.
The term used to describe the biochemical and morphologic changes that occur when blood is stored at 1-6 degrees C :
Storage lesion
IgG sensitized cells are also called?
Check cells
In an antibody identification panel, only one red cell was negative at the antihuman globulin phase. On ruling out and matching the pattern, an anti-k was identified. What further testing is necessary to confirm the antibody?
Two more k-negative cells should be tested.
What is the next step in the investigating a positive DAT after testing with polyspecific AHG?
Repeat the direct antiglobulin test using monospecific anti-IgG and anti-C3 reagents.
Anti-D reagent and the D control were tested with patient’s red cells. Both tests were 2+ agglutination reactions. What is the interpretation of the results?
Unable to interpret without further testing/ Invalid
The weak D test detects:
A weak D antigen.
Predict the agglutination reaction of red cells from a Bombay phenotype when combined with anti-H lectin.
Negative
A group A man marries a group AB woman. The father of the group A man was group O. What possible ABO phenotypes could be expected in the offspring?
Group A, B and AB
A recipient with group A phenotype requires a transfusion of 2 units of frozen plasma. Which types are appropriate to select for transfusion?
AB and A
How many molecules of IgM are needed to activate complement?
1
A trauma patient with type AB is seen at a rural hospital. The hospital has only 3 units AB RBC's. What blood type of RBC's can the patient receive as an alternative?
Any
Does anti A2 exist?
No-A1 phenotype do not respond immunologically when exposed to A2 red cells.
Which blood type has the most H antigens?
O __ As a result, group O red cells carry no A or B antigens but are rich in unconverted H antigens.
Which transferase does A allele produce?
N-acetylgalactosaminyltransferase The A allele produces N-acetylgalactosaminyltransferase, which transfers the sugar, N-acetylgalactosamine, to an
What conditions are associated with acquired B antigen?
Group A1 individuals with disease of lower GI tract
Do ABO antibodies require the use of potentiators in vitro?
NO
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