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Chromatography HPLC

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  • An example limitation of HPLC
    Expensive
  • HPLC technique that separates molecules based on charge differences.
    Ion Exchange
  • The stationary material packed inside the column.
    Stationary Phase
  • The most common HPLC mode where the stationary phase is nonpolar and the mobile phase is polar.
    Reverse Phase
  • HPLC technique that separates molecules based on molecular size.
    Size Exclusion
  • The liquid that carries the sample through the column.
    Mobile Phase
  • Detects very small concentrations of a substance with high sensitivity.
    High Sensitivity
  • Advantage of HPLC compared to Gas Chromatography for proteins and vitamins.
    Works with Heat-Sensitive Compounds
  • One major application of HPLC in pharmaceuticals.
    Drug Quality Control
  • The HPLC mode where polar compounds are retained longer because the stationary phase is polar.
    Normal Phase
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