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Blood Bank Recap - Chapter 5,6 and 7

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  • A description of the antiglobulin test
    Human globulin is injected into an animal
    Human globulin is eluted from RBCs during saline washing
    AHG reacts with human globulin molecules bound to rbcs
    IgG and C3d are required for RBC sensitization
  • Rh antibodies are primarily of which immunoglobulin class
    IgD
    IgA
    IgG
    IgM
  • RBCs must be washed in saline at least three times before the addition of AHG reagent to:
    Neutralize any excess AHG reagent
    Remove traces of free serum globulins
    Increase antibody binding to antigen
    Wash away any hemolyzed cells.
  • Cells carrying a weak D require the use of what test to demonstrate its presence
    Warm Autoadsorption
    IAT
    Microplate Test
    DAT
  • If a patient who is R1R1 is transfused with a donor who is R0r, which antibody is he most likely going to produce?
    Anti-e
    Anti-c
    Anti-D
    Anti-G
  • What are the possible ABO phenotypes of the offspring from the mating of a group A to a group B individual?
    A, B, AB
    O, A, B
    A, B
    O, A, B, AB
  • An ABO type on a patient gives the following results: Anti-A: 4+; Anti-B: 4+; Anti-A1: 0 / A1 cells: 2+; B cells: 0 These reactions may be seen in a patient who is:
    AB with increased concentrations of protein in the serum
    A2B with an anti-A1
    AB with an autoantibody
    A1 with acquired B