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Bacterial Genetics
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Why are genes regulated?
To conserve energy, respond to the environment.
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What codon is the "start" codon?
AUG/Methionine
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What is a promoter?
The site where RNA Polymerase binds, signaling the beginning of transcription.
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What are the components of a nucleotide?
A phosphate, a pentose and a nitrogenous base
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I have genes for both green eyes and blue eyes. Is this my genotype or my phenotype?
Genotype
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If B. cereus is 30% cytosine, what percentage of it is thymine?
20%
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What are some characteristics of RNA?
Single stranded. Ribose as the pentose. Uracil is unique to RNA.
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What is the function of single strand binding proteins?
Maintain the integrity of the replication fork. Holds it open
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These are the nitrogenous bases found within DNA.
Adenine, Guanine, Cytosine and Thymine
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This kind of bond forms the backbone of both DNA and RNA
Phosphodiester bonds.
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Describe the central dogma theory.
The flow of genetic information: DNA -> RNA -> Protein.
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What is a Gene?
A specific sequence of nucleotides that code for RNA molecules or proteins.
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What are some characteristics of DNA?
Double stranded. Deoxyribose as the pentose. Thymine is unique to DNA.
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This nitrogenous base is found only in RNA
Uracil
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This enzyme divides double stranded DNA during replication
Helicase
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This enzyme forms a phosphodiester bond between new nucleotides.
Ligase
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What is a gene locus?
The specific location of a gene on a chromosome.
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If B. cereus has 27% Adenine in its DNA, what percent is composed of uracil?
There's no uracil in DNA ;)
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What is meant by "semiconservative" DNA replication?
Half of the new DNA strand is "conserved" from the parent strand, and the other half is newly replicated.
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These contain genetic information that isn't necessary for life, but may contain beneficial information, like drug resistance.
Plasmids
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What is a genome?
The complete genetic content of an organism
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