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PCR & RT-PCR
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3rd step in the PCR reaction when taq polymerase attaches and begins to ad nucleotides to create a new double-stranded piece of DNA at about 70°C.
Primer Extension
How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?
16
Polymerase used for PCR is extracted from _____________ .
Thermus aquaticus.
What is the process of binding of primer to the denatured strand called?
Annealing
Thermus aquatics is the source of _________.
Taq polymerase
The polymerase chain reaction is_________.
It is a DNA amplification technique
1st step in the PCR reaction, when double stranded DNA is heated at 95°C to separate into two pieces of single-stranded DNA
DNA denaturation
Short, single-stranded segments of DNA that are designed to be complementary to the beginning and end of the target sequence that will be amplified.
PCR Primers
Refers to the synthesis of DNA from an RNA template.
Reverse transcription (RT)
2nd step in the PCR reaction when the short DNA molecules called primers bind to a complimentary strand of the target deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) that to be copied. This happens at 55-65°C.
Annealing of primers
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