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List five factors affecting enzyme activity.
temperature, pH, enzyme concentration, substrate concentration, inhibitors
What is the complete name of the 3rd unit in your biology coursebook?
Enzymes
What is the independent variable for the last experiment you performed in the biology lab?
Substrate (H2O2) concentration
Inhibition of which nhibitor, X or Y, cannot be eased by increasing the substrate concentration?
Y
What is the relation of Km to Vmax
Km is the substrate concentration for 1/2 Vmax
What do competitive inhibitors decrease; Vmax only, Km only or both?
V max only
Which enzyme has lower affinity for its substrate?
B
Which of the enzymes in the table has the highest affinity for its substrate?
Lysosyme
This following graph will be used to calculate Km. Which variabl should be on the X axis?
Substrate concentration
You are asked to temperature vs volume of oxygen collected graph. Which variable goes to X axis?
Temperature
You are asked to plot time vs volume of oxygen collected graph. Which variable goes to X axis?
time
Diagram shows an ezperimental set up to investigate the effect of enzyme concentration on the rate of reaction.What is the dependent factor in this experiment?
Rate of reaction/ volume of oxygen(gas) collected
Where is the enzyme in this experiment?
Inside the potato (cubes)
What are the products in the reaction catalysed by catalase enzyme?
Water and oxygen
The activity of human enzymes stosp at very low temperatures. What is this called?
inactivation.
The graph shows how the concentration of components of an enzyme catalysed reaction changes with time. Which line represents the enzyme-substrate complexes?
D
Which hypothesis suggests that the shape of the enzyme changes slightly upon binding the substrate for better fit?
Induced fit
Which represents the enzyme, lock or key?
Lock
Which variable is on the X axis?
Temperature
What do enzymes lower to make rections go faster?
Activation energy
The graph shows energy changes in a chemical reaction. What is the activation energy when an enzyme is NOT added?
1+2
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