What microscope would use to view specimens 200nm to 10mm
light microscopes
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15
What lenses does light pass through in a compound microscope?
condenser lenses, objective lenses, ocular lens
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15
how are brightfield, darkfield, phase-contrast, and fluorescence microscopy similar?
They all need a light source
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15
What are helminths typically measured in?
millimeters
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15
how do acid fast negative bacteria appear after preforming an acid fast stain
no mycolic acids in cell wall so unable to retain carbolfuschin after decolorizing step. instead take up the counterstain (methylene blue) and appear blue
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15
What is a special stain? Name the 3 special stains in the slides and explain what they are used for
capsule stain, endospore stain and flagella stain...
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15
Explain why negative staining involves acidic dyes. Give an example of an acidic dye
uses acidic/anionic dye because its (-)charge is repelled by the (-) charge on the outside of the cell staining the background not the microbe/cell. Nigrosin
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15
Differentiate an acidic dye from a basic dye.
Basic dyes have a (+) charge; acidic dyes have a (-) charge; Basic dyes attract to (-) charged bacterial cell wall due to their (+) charge
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15
What is another word for micrometers
micron
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15
differentiate gram staining and endospore staining
They both use 2 stains to differentiate microbes; differentiates between Gram(+)(-) appear purple/pink; endospore stain visulaizes endospore green in pink cell
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15
List Gram stain steps, and describe the appearance of gram-positive and gram-negative cells after each step.
