how do acid fast positive bacteria appear after preforming the acid fast stain
acid fast retain the primary stain after decolorization with acid-alcohol due to the resistant nature of their cell walls. (mycolic acid)
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15
What microscope would you use to view living bacteria in motion?
brightfield, darkfield, phase-contrast
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15
What microscope would you use to view the surface details of a virus?
SEM, AFM
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15
Define total magnification and resolution.
Total magnification - objective lens x ocular lens. The ocular lens is set to 10x. Resolution - the ability to distinguish two objects from eachother
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15
how do acid fast negative bacteria appear after preforming an acid fast stain
no mycolic acids in cell wall so unable to retain carbolfuschin after decolorizing step. instead take up the counterstain (methylene blue) and appear blue
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seesaw
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seesaw
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fairy
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baam
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15
What are helminths typically measured in?
millimeters
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thief
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shark
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shark
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baam
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lifesaver
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fairy
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banana
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15
Why is fixing necessary for most staining procedures?
helps preserve morphology, ensures the little beasties will not run off or be washed off the slide
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15
Why is the Gram stain so useful?
helps to differentiate between gram (+) and gram (-) also can determine morphology which can help determine further physiological tests
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15
What microscope would you use to view a ribosome
TEM
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15
What SEM stand for and what is it used for?
Scanning Electron Microscopy - Scans the surface. Produces 3D images
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15
how are brightfield, darkfield, phase-contrast, and fluorescence microscopy similar?
They all need a light source
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seesaw
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rocket
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rocket
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baam
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15
differentiate gram staining and endospore staining
They both use 2 stains to differentiate microbes; differentiates between Gram(+)(-) appear purple/pink; endospore stain visulaizes endospore green in pink cell
