Why do electron microscopes have greater resolution than light microscopes?
Electrons have a sgorter wavelength than visible light. Produces a higher resolution image than light microscopes
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15
What SEM stand for and what is it used for?
Scanning Electron Microscopy - Scans the surface. Produces 3D images
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15
Explain how the phase-contrast microscope works and what we use it for
Brings direct and reflected or diffracted light rays together to form an image of the specimen on the ocular lens. used for detailed observation of living cell
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15
What lenses does light pass through in a compound microscope?
condenser lenses, objective lenses, ocular lens
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15
What microscope would you use to view the surface details of a virus?
SEM, AFM
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15
Explain why negative staining involves acidic dyes. Give an example of an acidic dye
uses acidic/anionic dye because its (-)charge is repelled by the (-) charge on the outside of the cell staining the background not the microbe/cell. Nigrosin
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15
Which microscopes would you use to observe unstained cells?
Unstained cells are more productively observed using darkfield, phase-contrast, or DIC microscopy.
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15
Which type of microscopy is most likley used to observe stained cells?
Bright field microscopy
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15
How are electron microscopy and light microscopy different?
electron microscopes use a beam of electrons instead of a beam of light. Electrons have a shorter wavelength than visible light. Higher resolution than light
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15
Why is the Gram stain so useful?
helps to differentiate between gram (+) and gram (-) also can determine morphology which can help determine further physiological tests
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15
differentiate gram staining and endospore staining
They both use 2 stains to differentiate microbes; differentiates between Gram(+)(-) appear purple/pink; endospore stain visulaizes endospore green in pink cell
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15
Define total magnification and resolution.
Total magnification - objective lens x ocular lens. The ocular lens is set to 10x. Resolution - the ability to distinguish two objects from eachother
