Components that are ______ _________ in the mobile phase will travel further up the plate, or faster through the column. Itโs these differences in solubility and retention by the stationary phase that separate out the different substances.
more soluble
Oops!
Check
Okay!
Check
15
The mobile phase moves ________ or over the stationary phase.
through
Oops!
Check
Okay!
Check
25
Look at this diagram of a chromatogram produced using one-way chromatography on a mixture of substances A and B. Calculate the Rf value of spot A.
Rf value = Distance travelled by solvent Distance travelled by spot, Rf value of spot A = 7 รท 8 = 0.875
Oops!
Check
Okay!
Check
25
Explain what is meant by the terms โmobile phaseโ and โstationary phaseโ.
In all types of chromatography, two phases are present: a stationary phase, which does not move, and a mobile phase, which flows over the stationary phase.
Oops!
Check
Okay!
Check
10
A ______________ where the molecules can move. This is always a liquid or a gas.
mobile phase
Oops!
Check
Okay!
Check
20
In _________ _______________ the Mobile Phase is a Gas
Gas Chromatography
Oops!
Check
Okay!
Check
20
In _________ chromatography a solvent such as ethanol (the mobile phase), moves over a piece of paper (the stationary phase).
paper
Oops!
Check
Okay!
Check
heart
Other team wins 20 points!
Oops!
gift
Win 15 points!
Okay!
fairy
Take points!
5
10
15
20
25
lifesaver
Give 20 points!
Oops!
15
Which type of the chromatography?
One-way chromatography (or paper chromatography)
Oops!
Check
Okay!
Check
15
The distance each substance moves up the plate depends on its _________in the mobile phase and its retention by or adsorption to the stationary phase__
solubility
Oops!
Check
Okay!
Check
15
Oops!
Check
Okay!
Check
15
GC can be used to detect the presence and quantity of alcohol in the blood or urine samples of suspected drink-drivers. What do the letters GC stand for?
Gas chromatography. Different substances have different retention times
Oops!
Check
Okay!
Check
monster
Reset all scores!
Oops!
fairy
Take points!
5
10
15
20
25
rocket
Go to first place!
Okay!
lifesaver
Give 15 points!
Oops!
baam
Lose 20 points!
Oops!
fairy
Take points!
5
10
15
20
25
fairy
Take points!
5
10
15
20
25
thief
Give points!
5
10
15
20
25
25
HPLC is Done Under _________ _____________
High Pressure
Oops!
Check
Okay!
Check
10
A ___________where the molecules can't move. This must be a solid, or a liquid on a solid support.