What type of bond holds together Carbon and oxygen atoms in methanol
Single covalent
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shark
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15
Which microscopes give a 3D image of a specimen?
confocal and electron microscopes
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25
What is a basic dye? What is an acidic dye?
Basic- chromophore is a cation, basic dye (+) charged, is attracted to the (-) charged cell wall; acidic dyes are (-), chromophore is an anion, repels (-) cell
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25
What type of bond holds Li+ and Cl- in LiCl together
ionic
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15
What is the difference between an Ionic bond and a covalent bond?
Ionic is losing and gaining electron(s) Covalent is sharing the same electron(s)
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25
What dyes are used in an endospore stain? What is the procedure? If endospores are present, how do they appear?
primary stain of malachite green with heat, decolorize with water, counterstain with safranin, endospores will be green in red cell
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15
How are flagella stained?
use mordant on flagella and stain with carbolfuchsin
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gift
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25
What two functional groups are in all amino acids?
amine (âNH2) and carboxylic acid (âCOOH) functional groups, linked to the same carbon atom.
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15
using the whiteboard draw the following functional groups; sulfhydyl, ester, ether, carboxyl
SH, COO C2OH4, COOH
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20
What functional group is found in DNA, participates in energy metabolism and is a carbon single bonded to 3 hydrogens
Methyl
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20
What functional group helps build organic acids, proteins and lipids, (COOH)
carboxyl
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20
Compare and contrast DNA and RNA differ?
RNA contains the sugar ribose, DNA deoxyribose, DNA double strand, RNA single, RNA uses uracil, DNA thyamine; they both use Adenine, cytosil and guanine
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25
What happens when acid fast bacteria are stained?
primary stain carbolfuchsin is retained during decolorization step because of cell walls waxy lipids, stays red after counterstain of methylene blue
