How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?
16
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15
What is the process of binding of primer to the denatured strand called?
Annealing
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15
2nd step in the PCR reaction when the short DNA molecules called primers bind to a complimentary strand of the target deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) that to be copied. This happens at 55-65°C.
Annealing of primers
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20
Short, single-stranded segments of DNA that are designed to be complementary to the beginning and end of the target sequence that will be amplified.
PCR Primers
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15
Thermus aquatics is the source of _________.
Taq polymerase
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baam
Lose 20 points!
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gift
Win 15 points!
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gift
Win 25 points!
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baam
Lose 25 points!
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20
Refers to the synthesis of DNA from an RNA template.
Reverse transcription (RT)
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15
3rd step in the PCR reaction when taq polymerase attaches and begins to ad nucleotides to create a new double-stranded piece of DNA at about 70°C.
Primer Extension
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15
Polymerase used for PCR is extracted from _____________ .
Thermus aquaticus.
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15
1st step in the PCR reaction, when double stranded DNA is heated at 95°C to separate into two pieces of single-stranded DNA